Cytoplasmic conductivity

WebDec 13, 2024 · In mammalian cells cytoplasm ion concentrations and hence cytoplasm conductivity is an important indicator of their physiological state. Changes in the cytoplasm conductivity has been... WebOur studies have revealed that K562 (human myelogenous leukemia) cells show a persistent elevation in the cytoplasmic conductivity occurring as early as 30 minutes following exposure to staurosporine. This method therefore allows a far more rapid detection method than existing biochemical marker methods.

Quantitative Model for Ion Transport and Cytoplasm Conductivity of ...

WebSep 2, 2024 · This formulation gives an average difference between model and measurement of 3.9% when compared to DEP-derived cytoplasm conductivities across all five treatment cases; and at three... WebMay 28, 2024 · Cells were centrifuged at room temperature at 1000 rpm for 5 min and washed in a ROT solution with the conductivity of 360 mS m … hillmeade nursing facility in nc https://healingpanicattacks.com

Cytoplasmic conductivity as a marker for bioprocess …

WebSep 2, 2024 · This could include the conductivity of cytoplasmic components which do not diffuse across the cell membrane, including proteins and ions with low permeability. … WebJun 30, 2024 · Amongst these methods, electrorotation measurement is considered to be one of the most accurate method in determining the dielectric properties of cells such as … WebDec 7, 2024 · In other words, the steady state ionic content of the cytoplasm, which determines cytoplasmic conductivity (σ cyt), is lowered by an increased net rate of ion efflux that occurs in isolated RBCs ... smart folder on iphone

Specific membrane capacitance, cytoplasm conductivity and

Category:Cytoplasmic anion/cation imbalances applied across the …

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Cytoplasmic conductivity

Determination of membrane capacitance and cytoplasm …

WebJun 30, 2024 · Amongst these methods, electrorotation measurement is considered to be one of the most accurate method in determining the dielectric properties of cells such as their membrane capacitance as well as conductivity and permittivity of cytoplasm (Yang et al. 1999). In this method, a rotational force is applied to cells by multiple dielectrophoretic ... WebJun 23, 2024 · A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting Scientific Reports Article Open Access Published: 23...

Cytoplasmic conductivity

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WebFeb 5, 2024 · Dielectric spectroscopy (DS) is a noninvasive technique for real-time measurements of the impedance spectra of biological cells. DS enables characterization of cellular dielectric properties such as membrane capacitance and cytoplasmic conductivity. We have developed a lab-on-a-chip device that uses an electro-activated microwells … WebDec 13, 2024 · In this work, a model that predicts the effects of physiological changes in ion transport on the cytoplasm conductivity of Chinese hamster ovary (CHO) cells is …

WebCytoplasmic conductivity of human erythrocytes may be significantly disturbed by the com- position of the external suspending media. Effects of external NaCl on cytoplasmic conduc- tivity of... WebJul 9, 2024 · In this work, we monitored the change in cytoplasm conductivity of Chinese hamster ovary (CHO) cells during nutrient deprivation and reintroduction. Employing single cell dielectrophoresis, the change in cytoplasm conductivity was measured over a 48-hr period. The conditions under which the cytoplasm conductivity would recover to …

WebMar 4, 2024 · Phenotypic quantification of cells based on their plasma membrane capacitance and cytoplasmic conductivity, as determined by their dielectrophoretic frequency dispersion, is often used as a marker for their biological function. However, due to the prevalence of phenotypic heterogeneity in many biological systems of interest, there … WebNov 14, 2024 · The cytoplasmic membrane integrity was analyzed by testing the relative electrical conductivity, leakage of intracellular substance, and permeation of fluorescent probe. The results indicated that the short-term adaptation improved the membrane integrity of yeast cell. The inhibition mechanism of phenolic acid might be attributed to the ...

WebDefine cytoplasmic. cytoplasmic synonyms, cytoplasmic pronunciation, cytoplasmic translation, English dictionary definition of cytoplasmic. n. The protoplasm enclosed by …

WebThese results indicate that as intrinsic bioelectrical markers, specific membrane capacitance and cytoplasmic conductivity can be used to classify tumor subtypes. Keywords: cytoplasmic conductivity; impedance flow cytometry; intrinsic bioelectrical marker; specific membrane capacitance; tumor subtype classification. Publication types hillmead shepton malletWebJun 6, 2015 · 3.3. Conductivity of the cytoplasm: regulation of the cell cytoplasm screening in response to electrical stimulation. Figure 7 shows the sensitivity of the induced EF at the membrane apex and substrate (figure 7a) and the cytoplasm (figure 7b) to the cytoplasm conductivity values. At the apex, a higher cytoplasm conductivity results … hillmed healthWebFeb 1, 2024 · The measured lysate conductivity drop of 8.22% is comparable with in situ cell measurements. In comparison, the conductivity of LB medium was measured to be 1.04 ± 0.05 S/m in this work, lower than the predicted cytoplasmic conductivity of live E. coli. Thus, the dead cells are expected to loss ions to the medium, leading to a drop of … smart folio 12.9WebNov 10, 2016 · Pollen becomes metabolic active prior germination, thus changes in membrane integrity and cytoplasmic conductivity should be detectable when comparing germinating active with inactive pollen … hillmeade road bowieWebApr 1, 2009 · For the cytoplasmic conductivity, we use an approximate median value from the literature of 0.5 S/m. Because of the difficulty associated with determining the cytoplasmic conductivity, we also performed fitting for values of 0.25 S/m and 1.0 S/m. In both cases, the extracted cell wall conductivities changed by less than 10% from the … hillmead road kings nortonWebFeb 14, 2024 · Specific membrane capacitance, cytoplasm conductivity and instantaneous Young's modulus of single tumour cells Authors Ke Wang 1 2 , Yang Zhao 1 3 , Deyong Chen 1 2 , Beiyuan Fan 1 2 , Yulan Lu 1 2 , Lianhong Chen 1 2 , Rong Long 4 , Junbo Wang 1 2 , Jian Chen 1 2 Affiliations hillmer orthopäde hannoverWebFeb 14, 2024 · In this study, single cells in suspension (adherent cells treated with trypsin) were aspirated through a microfluidic constriction channel at 25 °C, and the entry … hillmon grove baptist church