Fitc annexin

Author: ahpi

August undefined, 2024

WebFluorescein isothiocyanate (FITC)-coupled annexin V (Clontech, Palo Alto, CA or Roche Molecular Biomedicals, Indianapolis, IN) Annexin V-binding buffer: 10 mM HEPES (pH 7.4), 150 mM NaCl, 5 mM KCl, 1 mM MgCl 2, 1.8 mM CaCl 2. Propidium iodide stock solution: 100 μg/ml in 20 mM HEPES, pH 7.4. WebAnnexin V is a Ca 2+-dependent phospholipid binding protein. This protein can bind to a variety of phospholipids, but it has the highest affinity for phosphatidylserine (PS). The ApoDETECT Annexin V-FITC Kit binds to …

Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)

WebFITC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins … http://www.cyto.purdue.edu/archive/flowcyt/research/cytotech/apopto/data/chap16.htm chi health it problem

FITC Annexin V - BD Biosciences

WebThe Calbiochem ® Annexin V-FITC Apoptosis Detection Kit II utilizes annexin V conjugated to FITC to bind to phosphatidylserine on the outer surface of cells undergoing apoptosis. … WebThe Annexin V-FITC, Propidum Iodide (PI) Solution and the Compensation Control cells are provided ready to use. There are enough reagents to perform 100 assays and enough … WebApr 13, 2024 · The resulting cells were stained with the mixture of Annexin V-FITC (5 µL) and propidium iodide (PI) (10 µL) for 15 min, and the mixture was analyzed with flow … gotha series porcelain tile

Translational control of Bcl-2 promotes apoptosis of gastric carcinoma ...

BioLegend

WebThe Annexin V-FITC Kit can be used to detect phosphatidylserine on the outer leaflet of the cell membrane using flow cytometry. The Annexin V-FITC Kit is for research use only. Not for use in diagnostic procedures. 2 Summary Annexins are a family of calcium-dependent phospholipid-binding proteins. They are abundant in eukaryotic Web2. Dilute FITC-Annexin V at a concentration of 1 mg/ml in binding buffer and resuspend cells in 1 ml of this solution (prepare it freshly each time). 3. Incubate 10 min in the dark at RT. 4. Add to the cell suspension 0.1 ml of PI solution prior to analysis to give a final concentration of 1 mg/ml. 5. gotha shoppenWebProduct overview. Annexin V-FITC Apoptosis Staining / Detection Kit ab14085 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine … goth asian girl

"WebProtect from light. Wash cells twice with Flow Cytometry Staining Buffer or equivalent. Wash cells once with 1X Binding Buffer. Resuspend cells in 1X Binding Buffer at 1-5 x 10 6 … " - Fitc annexin

Fitc annexin

FITC conjugation of Annexin V - drmr.com

WebThe protocol suggests 10-20 µg per mg of Annexin V; for a first-time titration of FITC, try a range of 2 to 100 µg FITC per mg of Annexin V (for instance, 2, 5, 10, 40, 100 µg per … WebJul 8, 2012 · Hence, My 3-step approach to gating Annexin V data appropriately. Step 1: Display your Annexin V channel versus your Membrane Permeability Channel (for ease of typing, I’ll assume we’re using Annexin V FITC and PI) on the entire, ungated data set, and draw a region around the double negative (DN) fraction. Step 2: Using the DN region …

Did you know?

WebProtect from light. Wash cells twice with Flow Cytometry Staining Buffer or equivalent. Wash cells once with 1X Binding Buffer. Resuspend cells in 1X Binding Buffer at 1-5 x 10 6 cells/mL. Add 5 μL of fluorochrome-conjugated Annexin V to 100 μL of the cell suspension. Incubate 10-15 minutes at room temperature. WebGeneral description. Annexin V-FITC kit allows fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry. The AnnexinV-FITC kit …

WebAnnexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. WebPI will stain DNA in addition to annexin V-stained phosphatidylserine, which is characteristic for apoptosis at a later stage. Early apoptotic cells remain PI-negative. I used cisplatin (10µM) as ...

WebSep 3, 2009 · For FITC–annexin V binding assays, it is important to incorporate a positive control sample. This is usually a sample of the particular cell type treated with a known apoptotic stimulus ...

WebThe Annexin V Apoptosis Detection Kit includes Annexin V (Catalog #100-0329 or Catalog #100-0331 or Catalog #100-0333), Annexin V Binding Buffer (Catalog #100-0334), and 7-AAD (Catalog #75001). The kit is used for the combined detection of early-stage cell apoptosis (using Annexin V) and late-stage cell apoptosis or necrosis (using both …

WebApr 14, 2024 · F. Aplikasi diagnostik metode flow cytometry. Selain itu, pengembangan instrumen yang lebih sederhana dan terjangkau, serta peningkatan jumlah antibodi yang bermanfaat telah memfasilitasi flow cytometry dalam aspek diagnostik: Diagnosis leukemia/limfoma, dimana antigen permukaan leukosit diukur pada sel neoplastik. gotha shopWebStaining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes. Manufacturer: BD … gotha sensitivWebThe ApoDETECT Annexin V-FITC Kit provides all the reagents needed to detect the apoptotic cells. Annexin V is a Ca 2+ -dependent phospholipid binding protein. This protein can bind to a variety of phospholipids, but it … chi health kearney clinicWebThe Annexin V-FITC Kit has been developed for detection and discrimination of apoptotic, necrotic, and dead cells. Apoptotic cells are stained positively for Annexin V-FITC that binds to phosphotidylserine (PS), but are negative for staining with propidium iodide (PI). Dead cells are stained positive for chi health jobs omaha neWebApr 13, 2024 · To determine the induction of apoptosis, dual staining of HL-60 and ML-1 cells with fluoresceinated annexin V (annexin V-FITC) and propidium iodide (PI) was performed using FITC Annexin V Apoptosis Detection Kit I. After 24 h and 48 h of cell incubation with the tested agents, the cells were harvested and then washed twice with … goth ashtrayWebAnnexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for PS, and is useful for identifying apoptotic cells with exposed PS. Propidium Iodide (PI) is … gotha silk heftWebFITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a higher affinity for … gotha sonneborner strasse